Indikation
Misstänkt infektion. Ange närmare frågeställning på remissen.
Provmaterial
Blod från vänster hjärthalva (alt en bit mjältvävnad): se Blododling. Vävnadsbitar och/eller aspirat från misstänkta infektionshärdar.
Provtagning och transport
Vävnadsbitarna placeras var för sig i anaeroba transportburkar s k "T-rör" (fås från laboratoriet). Större bitar, främmande kroppar, implantat i steril burk (art. nr 163 19). Aspirat i 10 eller 1,5 mL Oxoid Isolator specialrör (finns på obduktion).
Metod
Odling med anrikning.
Vävnad koloniserad med normalflora (inkluderar lunga) odlas kvantitativt.
Referenser
- BSOP 17 Standard operating procedure: Investigation of tissues and biopsies.
- De Jongh DS et al. Postmortem bacteriology: a practical method for routine use. Am J Clin Pathol 1968;49:424-428.
- Johanson WG et al. Bacteriologic diagnosis of nosocomial pneumonia following prolonged mechanical ventilation. Am Rev Respir Dis 1988;137:259-264.
- Koneman EW, Davis MA. Postmortem bacteriology. 3. Clinical significance of microorganisms recovered at autopsy. Am J Clin Pathol 1974;61:28-40.
- Martinez OV et al. Postmortem bacteriology of cadaver tissue donors: an evaluation of blood cultures as an index of tissue sterility. Diagn Microbiol Infect Dis 1985;3:193-200.
- du Moulin GC, Paterson DG. Clinical relevance of postmortem microbiologic examination: a review. Human Pathology 1985;16:539-548.
- Pierce JR et al. Immediate postmortem cultures in an intensive care nursery. Pediatr Infect Dis 1984;3:510-513.
- Roberts FJ. The association of antimicrobial therapy with postmortem spleen culture in bacteremic patients. Am J Clin Pathol 1987;87:770-772.
- Roberts FJ. Procurement, interpretation and value of postmortem cultures. Eur J Clin Microbiol Infect Dis 1998;17:821-827.
- Silver H, Sonnenwirth AC. A practical and efficacious method for obtaining significant postmortem blood cultures. Am J Clin Pathol 1969;52:433-437.
- Vehmeyer SBW et al. A comparative study of blood and bone marrow cultures in cadaveric bone donation. J Hosp Infection 1999;43:305-308.
- Wilson SJ et al. Diagnostic utility of postmortem blood cultures. Arch Pathol Lab Med 1993;117:986-988.
- Zanen-Lim OG, Zanen HC. Postmortem bacteriology of the lung by printculture of frozen tissue. J Clin Pathol 1980;33:474-480.